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Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis

  • Klaas J. van Wijk
  • Jean-Benoit Peltier
  • Lisa Giacomelli
Part of the Methods in Molecular Biology book series (MIMB, volume 355)

Abstract

This chapter describes a simple protocol for large-scale chloroplast purification for proteome analysis. The protocol has not been tested for protein import activity but is optimized for chloroplast proteome yield and purity and minimal protein degradation.

Key Words

Chloroplast protein Arabidopsis thaliana purification 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Klaas J. van Wijk
    • 1
  • Jean-Benoit Peltier
    • 1
  • Lisa Giacomelli
    • 1
  1. 1.Department of Plant BiologyCornell UniversityIthaca

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