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Plant Proteomics and Glycosylation

  • Anne-Catherine Fitchette
  • Olivia Tran Dinh
  • Loïc Faye
  • Muriel Bardor
Part of the Methods in Molecular Biology book series (MIMB, volume 355)

Abstract

In plant cells, as in other eucaryotic cells, glycosylation is one of the most studied posttranslational events. It can be of two types, N- or O-glycosylation, depending on the linkage involved between the protein backbone and the oligosaccharide moiety. In this review, we present different methods, commonly used in our laboratory, to study the glycosylation of plant proteins. These approaches rely on blot detection with glycan-specific probes, as well as specific deglycosylation of the glycoproteins, followed by mass spectrometry analysis. Such experiments not only allow determination of whether the protein is a glycoprotein, but also how and where it is glycosylated. The last part of this chapter is dedicated to the specific purification and identification of glycoprotein populations in plant cells, so-called glycoproteomics.

Key Words

N-glycosylation O-glycosylation O-N-acetylglucosamine lectin anti-glycan antibodies β-elimination endoglycosidase H peptide-N-glycosidase F peptide-N-glycosidase A glycoproteomics MALDI-TOF LC-MS/MS 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Anne-Catherine Fitchette
    • 1
  • Olivia Tran Dinh
    • 1
  • Loïc Faye
    • 1
  • Muriel Bardor
    • 1
  1. 1.CNRS UMR 6037, IFRMP 23, GDR 2590Université de RouenMont Saint-AignanFrance

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