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Protein Extraction from Xylem and Phloem Sap

  • Julia Kehr
  • Martijn Rep
Part of the Methods in Molecular Biology book series (MIMB, volume 355)

Abstract

It is well known that phloem and xylem vessels transport small nutrient molecules over long distances in higher plants. The finding that proteins also occur in both transport fluids was unexpected, and the function of most of these proteins is not yet well understood. This chapter outlines how proteins can be obtained and purified from xylem and phloem saps to perform subsequent proteomic analyses.

Key words

Xylem sap phloem sap protein precipitation proteomics 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Julia Kehr
    • 1
  • Martijn Rep
    • 2
  1. 1.Max-Planck-Institute of Molecular Plant PhysiologyPotsdamGermany
  2. 2.Plant Pathology, Swammerdam Institute for Life SciencesUniversity of AmsterdamAmsterdamThe Netherlands

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