Multivariate Data Analysis of Proteome Data

  • Kåre Engkilde
  • Susanne Jacobsen
  • Ib Søndergaard
Part of the Methods in Molecular Biology book series (MIMB, volume 355)


We present the background for multivariate data analysis on proteomics data with a hands-on section on how to transfer data between different software packages. The techniques can also be used for other biological and biochemical problems in which structures have to be found in a large amount of data. Digitalization of the 2D gels, analysis using image processing software, transfer of data, multivariate data analysis, interpretation of the results, and finally we return to biology.

Key Words

Multivariate data analysis proteomics 2D-electrophoresis 2D-gels PCA PLSR image processing 


  1. 1.
    Gottlieb, D. M., Schultz, J., Bruun, S. W., Jacobsen, S., and Søndergaard, I. (2004) Multivariate approaches in plant science. Phytochemistry 65, 1531–1548.CrossRefPubMedGoogle Scholar
  2. 2.
    Jessen, F., Lametsch, R., Bendixen, E., Kjærsgård, I. V. H., and Jørgensen, B. M. (2002) Extracting information from two-dimensional electrophoresis gels by partial least squares regression. Proteomics 2, 32–35.CrossRefPubMedGoogle Scholar
  3. 3.
    Radzikowski, L., Nešić, L., Hansen, H. B., Jacobsen, S., and Søndergaard, I. (2002) Comparison of ethanol-soluble proteins from different rye (Secale cereale) varieties by two-dimensional electrophoresis. Electrophoresis 23, 4157–4166.CrossRefPubMedGoogle Scholar
  4. 4.
    Schultz, J., Gottlieb, D. M., Petersen, M., Nešíc, L., Jacobsen, S., and Søndergaard, I. (2004) Explorative data analysis of 2-D electrophoresis gels. Electrophoresis 25, 502–511.CrossRefPubMedGoogle Scholar
  5. 5.
    Martens, H. and Martens, M. (2001) Multivariate Analysis of Quality. An Introduction. John Wiley & Sons, New York.Google Scholar
  6. 6.
    Esbensen K. H. (2000) Multivariate Data Analysis—In Practice. Camo, Oslo, Norway.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Kåre Engkilde
    • 1
  • Susanne Jacobsen
    • 1
  • Ib Søndergaard
    • 1
  1. 1.BioCentrum-DTU, Biochemistry and Nutrition GroupTechnical University of DenmarkLyngbyDenmark

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