Abstract
The CellCard™ system enables the analysis of multiple cell types within a single microtiter well. In doing so, the CellCard system not only determines the effect of an experimental condition on a cell type of interest, but also the relative selectivity of that response across nine other cell types. In addition, this approach of cellular multiplexing is a means of miniaturization without the necessity of microfluidic devices. The standard 96-well plate generates ten 96-well plates of data (or, the equivalent of a 960-well plate). Taken together, the CellCard technology enables multiple cell types to be assayed within a single microtiter well allowing for the simultaneous determination of cellular activity and compound selectivity. This chapter will describe a method by which multiple cell types can be simultaneously assayed for biological parameters of interest.
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© 2007 Humana Press, Inc.
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Beske, O., Bassoni, D., Goldbard, S. (2007). Use of the CellCard™ System for Analyzing Multiple Cell Types in Parallel. In: Taylor, D.L., Haskins, J.R., Giuliano, K.A. (eds) High Content Screening. Methods in Molecular Biology, vol 356. Humana Press. https://doi.org/10.1385/1-59745-217-3:129
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DOI: https://doi.org/10.1385/1-59745-217-3:129
Publisher Name: Humana Press
Print ISBN: 978-1-58829-731-0
Online ISBN: 978-1-59745-217-5
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