Abstract
We outline a strategy for the optimization of buffer conditions for the solubilization, extraction, and isoelectric focusing (IEF) of proteins from cardiac tissue for two-dimensional gel electrophoresis (2DE). This strategy, which involves altering both the extraction and IEF buffers, allows one to ensure representation of the proteome that is as complete as possible. Initial buffer choices are given, as well as basic protocols for modifications. Although these conditions have been effectively demonstrated for human myocardium, in principle this procedure can be used for the initial screen of any new sample of tissue or cultured cells.
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© 2007 Humana Press Inc.
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Stanley, B.A., Van Eyk, J.E. (2007). A Solubility Optimization Protocol for Two-Dimensional Gel Electrophoresis of Cardiac Tissue. In: Vivanco, F. (eds) Cardiovascular Proteomics. Methods in Molecular Biology™, vol 357. Humana Press. https://doi.org/10.1385/1-59745-214-9:59
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DOI: https://doi.org/10.1385/1-59745-214-9:59
Publisher Name: Humana Press
Print ISBN: 978-1-58829-535-4
Online ISBN: 978-1-59745-214-4
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