Laser-Induced Thrombosis in Zebrafish Larvae

A Novel Genetic Screening Method for Thrombosis
  • Pudur Jagadeeswaran
  • Ryan Paris
  • Prashanth Rao
Part of the Methods in Molecular Medicine book series (MIMM, volume 129)


Classical genetic approaches to study hemostasis and thrombosis have not been available until our recent introduction of the teleost, Danio rerio (the zebrafish), as an effective genetic model for in vivo coagulation assays. The genetic screen for this model is carried out using the genome saturation mutagenesis approach. The resulting mutants are screened for hemostatic or thrombotic defects. We developed a global physiological screening method for thrombosis by utilizing a laser to induce thrombosis in a specifically targeted area of the major artery and vein. Using this assay, we have screened many fish for abnormal hemostasis, and have isolated a number of mutants with abnormal coagulation parameters. These mutants can be grown, bred, and further evaluated for the genetic etiology of their abnormal hemostatic pathways.

Key Words

Zebrafish hemostasis thrombosis laser coagulation in vivo screening assays thrombocytes mutagenesis 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Pudur Jagadeeswaran
    • 1
  • Ryan Paris
    • 1
  • Prashanth Rao
    • 1
  1. 1.Department of Cellular and Structural BiologyThe University of Texas Health Science Center at San AntonioSan Antonio

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