Abstract
Classical genetic approaches to study hemostasis and thrombosis have not been available until our recent introduction of the teleost, Danio rerio (the zebrafish), as an effective genetic model for in vivo coagulation assays. The genetic screen for this model is carried out using the genome saturation mutagenesis approach. The resulting mutants are screened for hemostatic or thrombotic defects. We developed a global physiological screening method for thrombosis by utilizing a laser to induce thrombosis in a specifically targeted area of the major artery and vein. Using this assay, we have screened many fish for abnormal hemostasis, and have isolated a number of mutants with abnormal coagulation parameters. These mutants can be grown, bred, and further evaluated for the genetic etiology of their abnormal hemostatic pathways.
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© 2006 Humana Press Inc.
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Jagadeeswaran, P., Paris, R., Rao, P. (2006). Laser-Induced Thrombosis in Zebrafish Larvae. In: Wang, Q.K. (eds) Cardiovascular Disease. Methods in Molecular Medicine, vol 129. Humana Press. https://doi.org/10.1385/1-59745-213-0:187
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DOI: https://doi.org/10.1385/1-59745-213-0:187
Publisher Name: Humana Press
Print ISBN: 978-1-58829-892-8
Online ISBN: 978-1-59745-213-7
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