Animal Models for Hypertension/Blood Pressure Recording
Hypertension is an important disease with polygenic inheritance. In order to identify the genes involved in blood pressure regulation, hypertensive rat and mouse models have been developed either by selective breeding or by transgenic technology. The most essential technological prerequisite in these studies is a reliable assessment of the blood pressure in rodents. Three methods are used most frequently for this purpose: tail cuff plethysmography, intra-arterial catheters, and radiotelemetry. Plethysmography is noninvasive, relatively simple, and suitable for a large number of animals, but also imprecise. Intra-arterial catheters are more precise, but require surgery. And both methods restrain and thereby stress the animals, which leads to alterations in blood pressure. Therefore, the telemetric blood pressure measurement, which allows the study of conscious, freely moving animals, has become the gold standard for measuring blood pressure in rodents. However, this method is extremely expensive. Thus, for each experiment the costs have to be put in relation to the quality of data required. This chapter will describe blood pressure measurement methods in technical detail.
Key WordsBlood pressure heart rate tail cuff plethysmography radiotelemetry tip catheter
- 4.Bader, M. and Ganten, D. (2004) Transgenics of the RAS, in Handbook of Experimental Pharmacology: Angiotensin, (Unger, T. and Schölkens, B. A., eds.), Springer Verlag, Heidelberg, Germany: pp. 229–249.Google Scholar
- 11.Kurtz, T. W., Griffin, K. A., Bidani, A. K., Davisson, R. L., and Hall, J. E. (2005) American Heart Association: recommendations for blood pressure measurement in humans and experimental animals. Part 2: blood pressure measurement in experimental animals: a statement for professionals from the subcommittee of professional and public education of the American Heart Association council on high blood pressure research. Hypertension 45, 299–310.CrossRefPubMedGoogle Scholar
- 17.Bader, M. (2005) Mouse knockout models for hypertension, in Hypertension: Methods and Protocols, (Fennell, J. and Baker, A. H., eds.), Humana Press, Totowa, NJ: pp. 17–32.Google Scholar