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Microarray Analysis of Cardiovascular Diseases

  • Stephen R. Archacki
  • Qing K. Wang
Part of the Methods in Molecular Medicine book series (MIMM, volume 129)

Abstract

Microarray analysis is a powerful technique for high-throughput, global transcriptonomic profiling of gene expression. It holds great promise for analyzing the genetic and molecular bases of cardiovascular diseases and various other complex diseases and permits the analysis of thousands of genes simultaneously, both in diseased and nondiseased tissues and/or cell lines. Microarrays or microchips are made by depositing spots of DNA or oligonucleotides representing thousands of genes on a solid support such as a coated glass surface, and can allow the comparison of gene expression patterns in any two samples. Total RNA is isolated from the tissue or cells of interest, converted to cDNA and then cRNA labeled with biotin, and hybridized to the chips. Hybridization signals are then quantified and compared among different samples. We used oligonucleotide microarrays to obtain an unbiased assessment of expression levels of thousands of genes simultaneously in normal and diseased coronary arteries. Fifty-six genes showed differential expression in atherosclerotic coronary artery tissues, and 49 of them represent new linked genes for coronary artery disease. These studies can generate novel hypotheses relating to the pathologies of disease and further studies with animal models, molecular biology, cell biology, and biochemistry will validate these hypotheses and provide novel insights into the pathogenesis of disease.

Key Words

Oligonucleotide array microarray, genechip expression profiling coronary artery disease gene genetics atherosclerosis 

References

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    Archacki, S. R. and Wang, Q. (2004). Expression profiling of cardiovascular disease. Human Genomics 1, 355–370.PubMedGoogle Scholar
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    Shen, G. Q., Archacki, S. R., and Wang, Q. (2004) The molecular genetics of coronary artery disease and myocardial infarction. Acute Coronary Syndrome 6, 129–141.Google Scholar
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    Archacki, S. R., Angheloiu, G., Tian, X. L., et al. (2003) Identification of new genes differentially expressed in coronary artery disease by expression profiling. Physiol. Genomics 15, 65–74.PubMedGoogle Scholar
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    You, S. A., Archacki, S. R., Angheloiu, G., et al. (2003) Proteomic approach to coronary atherosclerosis shows ferritin light chain as a significant marker: evidence consistent with iron hypothesis in atherosclerosis. Physiol. Genomics 13, 25–30.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Stephen R. Archacki
    • 1
  • Qing K. Wang
    • 2
    • 3
    • 4
  1. 1.Center for Molecular GeneticsThe Cleveland Clinic FoundationCleveland
  2. 2.Department of Molecular CardiologyThe Cleveland Clinic FoundationCleveland
  3. 3.Center for Cardiovascular GeneticsThe Cleveland Clinic FoundationCleveland
  4. 4.Department of Cardiovascular MedicineThe Cleveland Clinic FoundationCleveland

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