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DNA Vaccines pp 11–22Cite as

Design of Plasmid DNA Constructs for Vaccines

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 127))

Abstract

For more than three decades, plasmids have been widely used in the biotechnology arena. Historically, they have been most often employed for the expression of heterologous proteins in a variety of microorganisms. More recently, plasmids have been used as vectors for the delivery of antigen encoding genes in order to elicit immune responses in higher order animals. In this chapter, we discuss methods for constructing vectors with this unique purpose. Considerations for choosing the replicon, antigen, expression elements, and host cells are discussed within the context of developing a commercially viable vaccine vector.

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Author information

Authors and Affiliations

  1. Vaccines and Biologics Research, Merck Research Laboratories, West Point, PA

    Donna L. Montgomery

  2. Bioprocess Research and Development, Merck Research Laboratories, Rahway, NJ

    Kristala Jones Prather

Authors
  1. Donna L. Montgomery
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  2. Kristala Jones Prather
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Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, Yale University, New Haven, CT

    W. Mark Saltzman

  2. Department of Chemical Engineering, University of Washington, Seattle, WA

    Hong Shen

  3. Section of Comparative Medicine, Yale University, New Haven, CT

    Janet L. Brandsma

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© 2006 Humana Press Inc.

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Montgomery, D.L., Prather, K.J. (2006). Design of Plasmid DNA Constructs for Vaccines. In: Saltzman, W.M., Shen, H., Brandsma, J.L. (eds) DNA Vaccines. Methods in Molecular Medicine™, vol 127. Humana Press. https://doi.org/10.1385/1-59745-168-1:11

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  • DOI: https://doi.org/10.1385/1-59745-168-1:11

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-484-5

  • Online ISBN: 978-1-59745-168-0

  • eBook Packages: Springer Protocols

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