Abstract
Encapsulating DNA within degradable delivery vehicles such as micro- or nanospheres provides an effective way to protect the DNA from the surrounding environment prior to delivery. The ability to target these vehicles directly to the cell type of interest provides a way to enhance the overall efficiency of DNA delivery. One means of highly specific cell targeting is through the addition to the vehicle surface of ligands that bind specifically to receptors on the surface of the targeted cell type. Covalent conjugation of ligands to the surface of degradable delivery vehicles can be difficult, as the most commonly used vehicle formulations use materials selected for their general chemical inertness. This chapter describes methods for overcoming this, enabling encapsulation of DNA within degradable microspheres made of a commonly used biomaterial and then covalently conjugating ligands to the surface of these microspheres.
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References
Foster, N., Clark, M. A., Jepson, M. A., and Hirst, B. H. (1998) Ulex europaeus 1 lectin targets microspheres to mouse Peyer’s patch M-cells in vivo. Vaccine 16, 536–541.
Hermanson, G. T. (1996) Bioconjugate Techniques. Academic Press: New York, NY.
Scholes, P. D., Coombes, A. G. A., Illum, L., et al. (1999) Detection and determination of surface levels of poloxamer and PVA surfactant on biodegradable nanospheres using SSIMS and XPS. J. Control. Release 59, 261–278.
Blanco, M. D. and Alonso, M. J. (1997) Development and characterization of protein-loaded poly(lactide-co-glycolide) nanospheres. Eur. J. Pharm. Biopharm. 43, 287–294.
Cleland, J. L., Lim, A., Barron, L., Duenas, E. T., and Powell, M. F. (1997) Development of a single-shot subunit vaccine for HIV-1: Part 4. Optimizing microencapsulation and pulsatile release of MN rgp120 from biodegradable microspheres. J. Control. Release 47, 135–150.
Eniola, A. O., Rodgers, S. D., and Hammer, D. A. (2002) Characterization of biodegradable drug delivery vehicles with the adhesive properties of leukocytes. Biomaterials 23, 2167–2177.
O’Donnell, P. B. and McGinity, J. W. (1997) Preparation of microspheres by the solvent evaporation technique. Adv. Drug Deliver. Rev. 28, 25–42.
Yang, Y.-Y., Chung, T.-S., and Ng, N. P. (2001) Morphology, drug distribution, and in vitro release profiles of biodegradable polymeric microspheres containing protein fabricated by double-emulsion solvent extraction/evaporation method. Biomaterials 22, 231–241.
Liley, P. E., Thomson, G. H., Friend, D. G., Daubert, T. E., and Buck, E. (1997) Physical and chemical data. In: Perry’s Chemical Engineer’s Handbook (Perry, R. H., Green, D. W., and Maloney, J. O., eds.), McGraw-Hill, New York, NY, pp. 2-1–2-374.
Fahmy, T. M., Samstein, R. M., Harness, C., and Saltzman, W. M. (2005) Sustained target presentation in biodegradable polymers by surface modification with fatty acid conjugates. Biomaterials 26, 5727–5736.
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Keegan, M.E., Saltzman, W.M. (2006). Surface-Modified Biodegradable Microspheres for DNA Vaccine Delivery. In: Saltzman, W.M., Shen, H., Brandsma, J.L. (eds) DNA Vaccines. Methods in Molecular Medicine™, vol 127. Humana Press. https://doi.org/10.1385/1-59745-168-1:107
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DOI: https://doi.org/10.1385/1-59745-168-1:107
Publisher Name: Humana Press
Print ISBN: 978-1-58829-484-5
Online ISBN: 978-1-59745-168-0
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