Production of siRNA and cDNA-Transfected Cell Arrays on Noncoated Chambered Coverglass for High-Content Screening Microscopy in Living Cells

  • Holger Erfle
  • Rainer Pepperkok
Part of the Methods in Molecular Biology™ book series (MIMB, volume 360)


In this chapter, we provide a protocol for the production of transfected cell arrays in living mammalian cells on noncoated chambered coverglass for the systematic functional analyses of human genes by high-content screening microscopy. This method should facilitate drug target validation by small-interfering RNAs.

Key Words

Cell arrays genomics high-content screening microscopy small-interfering RNAs siRNA 


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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Holger Erfle
    • 1
  • Rainer Pepperkok
    • 1
  1. 1.Cell Biology and Cell Biophysics UnitEuropean Molecular Biology LaboratoryHeidelbergGermany

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