Skip to main content

Ginseng (Panax ginseng)

  • Protocol

Part of the book series: Methods in Molecular Biology ((MIMB,volume 344))

Abstract

Genetic transformation is an attractive way to improve Panax ginseng C. A. Meyer by introducing new genetic materials and altering metabolic pathways that regulating the production of secondary compounds. In P. ginseng, production of transgenic plants via Agrobacterium tumefaciens is performed via somatic embryogenesis using cotyledon or embryogenic callus as explants. This chapter introduces the protocol of A. tumefaciens mediated-genetic transformation in P. ginseng.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Vogler, B.K., Pittler, M.H., and Ernst, E. (1999) The efficacy of ginseng. A systematic review of randomized clinical trials. Eur. J. Clin. Pharmacol. 55, 567–575.

    Article  PubMed  CAS  Google Scholar 

  2. Ellis, J.M. and Reddy, P. (2002) Effects of Panax ginseng on quality of life. Ann. Pharmacother. 36, 375–379.

    Article  PubMed  Google Scholar 

  3. Yoshikawa, T. and Furuya, T. (1987) Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Rep. 6, 449–453.

    CAS  Google Scholar 

  4. Lee, H.S., Kim, S.W., Lee, K.W., Eriksson, T., and Liu, J.R. (1995) Agrobacterium-mediated transformation of ginseng (Panax ginseng) and mitotic stability of the inserted beta-glucuronidase gene in regenerants from isolated protoplasts. Plant Cell Rep. 14, 545–549.

    Article  CAS  Google Scholar 

  5. Yang, D.C. and Choi, Y.E. (2000) Production of transgenic plants via Agrobacterium rhizogenes-mediated transformation of Panax ginseng. Plant Cell Rep. 19, 491–496.

    Article  CAS  Google Scholar 

  6. Choi, Y.E., Yang, D.C., Kusano, T., and Sano, H. (2001) Rapid and efficient Agrobacterium-mediated genetic transformation by plasmolyzing pretreatment of cotyledons in Panax ginseng. Plant Cell Rep. 20, 616–621.

    Article  CAS  Google Scholar 

  7. Ohta, S., Mita, S., Hattori, T., and Nakamura, K. (1990) Construction and expression in tobacco of a β-glucuronidase (GUS) reporter gene containing an intron within the coding sequence. Plant Cell Physiol. 31, 805–813.

    CAS  Google Scholar 

  8. Choi, Y.E., Jeong, J.H., In, J.K., and Yang, D.C. (2003) Production of herbicide-resistant transgenic Panax ginseng through the introduction of phosphinotricin acetyl transferase gene and successful soil transfer. Plant Cell Rep. 21, 563–568.

    Article  PubMed  CAS  Google Scholar 

  9. Lee, M.H., Jeong, J.H., Seo, J.W., et al. (2004) Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene. Plant Cell Physiol. 45, 976–984.

    Article  PubMed  CAS  Google Scholar 

  10. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue. Physiol. Plant. 15, 473–497.

    Article  CAS  Google Scholar 

  11. Choi, Y.E., Yang, D.C., Park, J.C., Soh, W.Y., and Choi, K.T. (1998) Regenerative ability of somatic single and multiple embryos from cotyledons of Korean ginseng on hormone-free medium. Plant Cell Rep. 17, 544–551.

    Article  CAS  Google Scholar 

  12. Van Larebeke, N., Genetello, C., Hernalsteens, J.P., et al. (1977) Transfer of Ti plasmids between Agrobacterium strains by mobilization with the conjugative plasmid RP4. Mol. Gen. Genet. 152, 119–124.

    Article  Google Scholar 

  13. Kuribayashi, T. and Ohashi, H. (1971) Physiological and ecological studies in Panax ginseng. II. Effects of various temperature and chemical control substances on the germination. Syoyakugaku Zasshi 25, 95–101.

    CAS  Google Scholar 

  14. Choi, Y.E., Kim, H.S., Yang, D.C., and Soh, W.Y. (1997) Developmental and structural aspects of somatic embryos formed on medium containing 2,3,5-triiodobenzoic acid. Plant Cell Rep. 16, 738–744.

    Article  CAS  Google Scholar 

  15. Choi, Y.E., Yang, D.C., Yoon, E.S., and Choi, K.T. (1999) High efficiency plant production via direct somatic single embryogenesis from pre-plasmolysed cotyledons of Panax ginseng and possible dormancy of somatic embryos. Plant Cell Rep. 18, 493–499.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2006 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Choi, Y.E. (2006). Ginseng (Panax ginseng). In: Wang, K. (eds) Agrobacterium Protocols Volume 2. Methods in Molecular Biology, vol 344. Humana Press. https://doi.org/10.1385/1-59745-131-2:361

Download citation

  • DOI: https://doi.org/10.1385/1-59745-131-2:361

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-843-0

  • Online ISBN: 978-1-59745-131-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics