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Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation

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MicroRNA Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 342))

Abstract

MicroRNAs (miRNAs), an abundant class of approx 22-nucleotide (nt) small RNAs that control gene expression at the posttranscriptional level, may play important roles during normal hematopoiesis and leukemogenesis. This chapter focuses on the methods and strategies for dissecting miRNA function during hematopoietic lineage differentiation. We describe a modified miRNA cloning method and expression analysis approach for determining miRNA expression during hematopoietic lineage differentiation. We illustrate a retroviral vector and a general strategy for the ectopic expression of miRNAs in hematopoietic stem/progenitor cells. We discuss in vitro and in vivo functional assays that can be used to examine the roles of miRNAs during hematopoietic lineage differentiation. The methods and principles described here should also be applicable to study the roles of miRNAs in the differentiation and function of nonhematopoietic cell types.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Baxter Laboratory of Genetic Pharmacology, Institute for Stem Cell, Cancer and Medicine, Stanford University School of Medicine, Stanford, CA

    Hyeyoung Min & Chang-Zheng Chen

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  1. Hyeyoung Min
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  2. Chang-Zheng Chen
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Editors and Affiliations

  1. Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    Shao-Yao Ying

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© 2006 Humana Press Inc.

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Min, H., Chen, CZ. (2006). Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation. In: Ying, SY. (eds) MicroRNA Protocols. Methods in Molecular Biology™, vol 342. Humana Press. https://doi.org/10.1385/1-59745-123-1:209

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  • DOI: https://doi.org/10.1385/1-59745-123-1:209

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-581-1

  • Online ISBN: 978-1-59745-123-9

  • eBook Packages: Springer Protocols

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