Abstract
Models to study molecular, biochemical, and functional responses in vitro generally incorporate an individual cell type or group of cells organized in a random fashion. Normal physiological responses in vivo require that individual cell types be oriented in an organized fashion with three-dimensional architecture and appropriately positioned cellular interfaces. Much recent progress has been made in the development and implementation of models to study Cell-Cell contact using substrate grown cells. Here, we summarize the use of membrane permeable sup-ports to study functional responses in appropriately positioned cell types. These models incorpo-rate two or more different cells cultured in physiologically positioned locales on solid substrates. Models incorporating nonadherent cells (e.g., leukocytes) in co-culture with such models also are discussed. Such models have been used extensively to discovery both cell-bound as well as soluble mediators of physiological and pathophysiological processes.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Barrett, K. E. and Keely, S. J. (2000) Chloride secretion by the intestinal epithe-lium: molecular basis and regulatory aspects. Annu. Rev. Physiol. 62, 535–572.
Colgan, S. P., Furuta, G. T., and Taylor, C. T. Cytokines and epithelial function, in Microbial Pathogeneis and the Intestinal Epithelial Cell (Hecht, G., ed.), ASM Press Washington, DC, pp. 61–78.
Blume, E. D., Taylor, C. T., Lennon, P. F., Stahl, G. L., and Colgan, S. P. (1998) Activated endothelial cells elicit paracrine induction of epithelial chloride secretion: 6-keto-PGF1a is an epithelial secretagogue. J. Clin. Invest. 102, 1161–1172.
Pober, J. S. and Cotran, R. S. (1990) Overview: the role of endothelial cells in inflammation. Transplantation 50, 537–541.
Vane, J. R., Anggard, E. E., and Botting, R. M. (1990) Regulatory functions of the vascular endothelium. N. Eng. J. Med. 323, 27–36.
Berschneider, H. M. and Powell, D. W. (1992) Fibroblasts modulate intestinal secretory responses to inflammatory mediators. J. Clin. Invest. 89, 484–489.
Parkos, C. A., Colgan, S. P., Delp, C., Arnaout, M. A., and Madara, J. L. (1992) Neutrophil migration across a cultured epithelial monolayer elicits a biphasic resis-tance response representing sequential effects on transcellular and paracellular pathways. J. Cell. Biol. 117, 757–764.
Parkos, C. A., Delp, C., Arnaout, M. A., and Madara, J. L. (1991) Neutrophil migra-tion across a cultured intestinal epithelium: dependence on a CD11b/CD18-mediated event and enhanced efficiency in the physiologic direction. J. Clin. Invest. 88, 1605–1612.
Kaoutzani, P., Colgan, S. P., Cepek, K. L., et al. (1994) Reconstitution of cultured intestinal epithelial monolayers with a mucosal-derived T lymphocyte cell line: Modulation of epithelial phenotype dependent on lymphocyte-basolateral mem-brane apposition. J. Clin. Invest. 94, 788–796.
McKay, D. M. and Singh, P. K. (1997) Superantigen activation of immune cells evokes epithelial (T84) transport and barrier abnormalities via, I. F.N-gamma and, T. N.F-alpha: inhibition of increased permeability, but not diminished secretory responses by TGF-beta2. J. Immunol. 159, 2382–2390.
Bruyninckx, W. J., Comerford, K. M., Lawrence, D. W., and Colgan, S. P. (2001) Phosphoinositide 3-kinase modulation of beta(3)-integrin represents an endogenous "e;braking"e; mechanism during neutrophil transmatrix migration. Blood 97, 3251–3258.
Dharmsathaphorn, K. and Madara, J. L. (1990) Established intestinal cell lines as model systems for electrolyte transport studies. Methods Enzymol. 192, 354–389.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Humana Press Inc.
About this protocol
Cite this protocol
Louis, N.A., Daniels, D., Colgan, S.P. (2006). Cell-Cell Interactions on Solid Matrices. In: Colgan, S.P. (eds) Cell-Cell Interactions. Methods in Molecular Biology™, vol 341. Humana Press. https://doi.org/10.1385/1-59745-113-4:51
Download citation
DOI: https://doi.org/10.1385/1-59745-113-4:51
Publisher Name: Humana Press
Print ISBN: 978-1-58829-523-1
Online ISBN: 978-1-59745-113-0
eBook Packages: Springer Protocols