Summary
In this chapter, methods to isolate RNA and DNA from human leukocytes for the subsequent use in molecular diagnostic tests are described. In addition, protocols for cDNA synthesis are given, both for the use in conventional reverse transcription (RT)-polymerase chain reaction (PCR), and for the use in quantitative RT-PCR reactions. Because sensitive PCR methods are commonly used in molecular diagnostics, measures to avoid contamination are described. Finally, a simple procedure to control the quality of isolated RNA is described.
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© 2006 Humana Press Inc.
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Jansen, J.H., van der Reijden, B.A. (2006). Isolation of RNA and DNA From Leukocytes and cDNA Synthesis. In: Iland, H., Hertzberg, M., Marlton, P. (eds) Myeloid Leukemia. Methods In Molecular Medicine™, vol 125. Humana Press. https://doi.org/10.1385/1-59745-017-0:1
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DOI: https://doi.org/10.1385/1-59745-017-0:1
Publisher Name: Humana Press
Print ISBN: 978-1-58829-485-2
Online ISBN: 978-1-59745-017-1
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