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Reduced Uterine Perfusion Pressure (RUPP) Model for Studying Cardiovascular-Renal Dysfunction in Response to Placental Ischemia

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Placenta and Trophoblast

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 122))

Abstract

Despite being one of the leading causes of maternal death and a major contributor of maternal and perinatal morbidity, the mechanisms responsible for the pathogenesis of preeclampsia are unknown. The initiating event in preeclampsia has been postulated to be reduced uteroplacental perfusion. Placental ischemia/hypoxia is thought to lead to widespread activation/dysfunction of the maternal vascular endothelium, vasoconstriction and hypertension. Experimental induction of chronic uteroplacental ischemia appears to be the most promising animal model to study potential mechanisms of preeclampsia since reductions in uteroplacental blood flow in a variety of animal models lead to a hypertensive state that closely resembles preeclampsia in women. This chapter details the methods we use in our laboratory to produce the reduced uterine perfusion pressure (RUPP) model in the pregnant rat.

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References

  1. August, P. and Lindheimer, M. D. (1995) Pathophysiology of preeclampsia, in Hypertension, 2nd Edition (Laragh, J. L. and Brenner, B. M., eds.). Raven, New York: pp. 2407–2426.

    Google Scholar 

  2. Roberts, J. M., Taylor, R. N., Musci, T. J., Rodgers, G. M., Hubel, C. A., and McLaughlin, M. K. (1989) Preeclampsia: an endothelial cell disorder. Am. J. Obstet. Gynecol. 161, 1200–1204.

    CAS  PubMed  Google Scholar 

  3. Alexander, B. T., Bennett, W. A., Khalil, R. A., and Granger, J. P. (2001) Pathophysiology of pregnancy-induced hypertension. News Physiol. Sci. 16, 282–286.

    CAS  PubMed  Google Scholar 

  4. Granger, J. P., Alexander, B. T., Bennett, W. A., and Khalil, R. A. (2001) Pathophysiology of pregnancy-induced hypertension. Am. J. Hypertens. 14, 178–185.

    Article  Google Scholar 

  5. Granger, J. P., Alexander, B. T., Llinas, M. T., Bennett, W. A., and Khalil, R. A. (2001) Pathophysiology of hypertension during preeclampsia: Linking placental ischemia with endothelial dysfunction. Hypertension 38, 718–722.

    CAS  PubMed  Google Scholar 

  6. Fisher, S. J. and Roberts, J. M. (1999) Defects in placentation and placental perfusion, in Chesley’s Hypertensive Disorders in Pregnancy, 2nd Edition (Linhheimer, M. D., Roberts, J. M., and Cunningham, F. G., eds.). Appleton & Lange, Stanford, CT: pp. 377–394.

    Google Scholar 

  7. Conrad, K. P. (1990) Animal models of pre-eclampsia: do they exist? Fetal Medicine Rev. 2, 67–88.

    Article  Google Scholar 

  8. Ogden, E., Hildebrand, G. J., and Page, E. W. (1940) Rise in blood pressure during ischemia of the gravid uterus. Proc. Soc. Exper. Biol. Med. 43, 49–51.

    Google Scholar 

  9. Hodari, A. A. (1967) Chronic uterine ischemia and reversible experimental “toxemia of pregnancy”. Am. J. Obstet. Gynecol. 97, 597–607.

    CAS  PubMed  Google Scholar 

  10. Abitbol, M. M., Gallo, G. R., Pirani, C. L., and Ober, W. B. (1976) Production of experimental toxemia in the pregnant rabbit. Am. J. Obstet. Gynecol. 124, 460–470.

    CAS  PubMed  Google Scholar 

  11. Abitbol, M. M., Pirani, C. L., Ober, W. B., Driscoll, S. G., and Cohen, M. W. (1976) Production of experimental toxemia in the pregnant dog. Obstet. Gynecol. 48, 537–548.

    CAS  PubMed  Google Scholar 

  12. Losonczy, G., Brown, G., and Venuto, R. C. (1992) Increased peripheral resistance during reduced uterine perfusion pressure hypertension in pregnant rabbits. Am. J. Med. Sci. 303, 233–240.

    Article  CAS  PubMed  Google Scholar 

  13. Cavanagh, D., Rao, P. S., Tung, K. S., and Gaston, L. (1974) Eclamptogenic toxemia: The development of an experimental model in the subhuman primate. Am. J. Obstet. Gynecol. 120, 183–196.

    CAS  PubMed  Google Scholar 

  14. Cavanagh, D., Rao, P. S., Tsai, C. C., and O’Connor, T. C. (1977) Experimental toxemia in the pregnant primate. Am. J. Obstet. Gynecol. 128, 75–85.

    CAS  PubMed  Google Scholar 

  15. Combs, C. A., Katz, M. A., Kitzmiller, J. L., and Brescia, R. J. (1993) Experimental preeclampsia produced by chronic constriction of the lower aorta: Validation with longitudinal blood pressure measurements in conscious rhesus monkeys. Am. J. Obstet. Gynecol. 169, 215–223.

    CAS  PubMed  Google Scholar 

  16. Eder, D. J. and McDonald, M. T. (1987–1988) A role for brain angiotensin II in experimental pregnancy-induced hypertension in laboratory rats. Clin. Exp. Hyper. Preg. B6, 431–451.

    Article  CAS  Google Scholar 

  17. Alexander, B. T., Kassab, S. E., Miller, M. T., et al. (2001) Reduced uterine perfusion pressure during pregnancy in the rat is associated with increases in arterial pressure and changes in renal nitric oxide. Hypertension 37, 1191–1195.

    CAS  PubMed  Google Scholar 

  18. Alexander, B. T., Rinewalt, A. N., Cockrell, K. L., Bennett, W. A., and Granger, J. P. (2001) Endothelin-A receptor blockade attenuates the hypertension in response to chronic reductions in uterine perfusion pressure. Hypertension 37, 485–489.

    CAS  PubMed  Google Scholar 

  19. Alexander, B. T., Cockrell, K. L., Sedeek, M., and Granger, J. P. (2001) Role of the renin-angiotensin system in meditating the hypertension produced by chronic reductions in uterine perfusion pressure in the pregnant rat. Hypertension 38, 742–745.

    CAS  PubMed  Google Scholar 

  20. Crews, J. K., Herrington, J. N., Granger, J. P., and Khalil, R. A. (2000) Decreased endothelium-dependent vascular relaxation during reduction of uterine perfusion pressure in pregnant rats. Hypertension 35, 367–372.

    CAS  PubMed  Google Scholar 

  21. Llinas, M. T., Alexander, B. T., Abram, S. R., Sedeek, M., and Granger, J. P. (2002) Enhanced production of thromboxane A2 in response to chronic reductions in uterine perfusion pressure in pregnant rats. Am. J. Hypertens. 15, 793–797.

    Article  CAS  PubMed  Google Scholar 

  22. Llinas, M. T., Alexander, B. T., Capparelli, M., Carroll, M. A., and Granger, J. P. (2004) Cytochrome P-450 inhibition attenuates hypertension induced by reductions in uterine perfusion pressure in pregnant rats. Hypertension 43, 623–628.

    Article  CAS  PubMed  Google Scholar 

  23. Alexander, B. T., Llinas, M. T., Kruckeberg, W. C., and Granger, J. P. (2004) Larginine attenuates hypertension in pregnant rats with reduced uterine perfusion pressure. Hypertension 43, 832–836.

    Article  CAS  PubMed  Google Scholar 

  24. Nienartowicz, A., Link, S., and Moll, W. (1989) Adaptation of the uterine arcade in rats during pregnancy. J. Develop. Physiol. 21, 101–108.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS

    Joey P. Granger, B. Babbette D. LaMarca, Kathy Cockrell, Mona Sedeek, Charles Balzi & Derrick Chandler

  2. Department of Obstetrics and Gynecology, University of Mississippi Medical Center, Jackson, MS

    William Bennett

Authors
  1. Joey P. Granger
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  2. B. Babbette D. LaMarca
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  3. Kathy Cockrell
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  4. Mona Sedeek
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  5. Charles Balzi
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  6. Derrick Chandler
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  7. William Bennett
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Editor information

Editors and Affiliations

  1. Institute of Maternal-Fetal Biology Division of Cancer and Developmental Biology Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS

    Michael J. Soares

  2. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS

    Joan S. Hunt (University Distinguished Professor, Vice Chancellor for Research) (University Distinguished Professor, Vice Chancellor for Research)

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© 2006 Humana Press Inc.

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Granger, J.P. et al. (2006). Reduced Uterine Perfusion Pressure (RUPP) Model for Studying Cardiovascular-Renal Dysfunction in Response to Placental Ischemia. In: Soares, M.J., Hunt, J.S. (eds) Placenta and Trophoblast. Methods in Molecular Medicine™, vol 122. Humana Press. https://doi.org/10.1385/1-59259-989-3:381

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  • DOI: https://doi.org/10.1385/1-59259-989-3:381

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-608-5

  • Online ISBN: 978-1-59259-989-9

  • eBook Packages: Springer Protocols

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