Establishment of an ELISA for the Detection of Native Bovine Pregnancy-Associated Glycoproteins Secreted by Trophoblast Binucleate Cells

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 122)


The pregnancy-associated glycoproteins (PAGs) are a large gene family expressed in trophoblast cells of ruminant ungulates. The detection of PAGs in maternal serum has served as the basis for pregnancy detection in ruminant ungulates and also for use as markers of trophoblast development and placental viability. The methods described provide a means for the rapid purification of bovine PAGs by affinity chromatography and the establishment of an enzyme-linked immunosorbent assay (ELISA) to measure PAG concentrations in maternal blood plasma and other biological fluids.

Key Words

Placenta trophoblast pregnancy pregnancy detection cattle 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  1. 1.Department of Animal ScienceUniversity of Missouri-ColumbiaColumbia
  2. 2.Life Sciences CenterUniversity of Missouri-ColumbiaColumbia

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