Summary
A method for producing high-molecular-weight DNA from pulverized tissue, nuclear fractions, or cultured cells. This isolation method relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent sodium dodecyl sulfate. Ethylenediaminetetraacetic acid is included in the lysis buffer to inhibit DNases.
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© 2006 Humana Press Inc.
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Robertson, N., Leek, R. (2006). Isolation of DNA From Tumor Samples. In: Brooks, S.A., Harris, A. (eds) Breast Cancer Research Protocols. Methods in Molecular Medicine, vol 120. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-969-9:61
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DOI: https://doi.org/10.1385/1-59259-969-9:61
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-191-2
Online ISBN: 978-1-59259-969-1
eBook Packages: Springer Protocols