Abstract
Ras signaling pathway is thought to control the expression of a subset of yet-to-be-defined genes that are crucial for cell growth and differentiation. Here we have identified by differential display a novel oncogenic Ras target gene encoding a new cytokine. Biochemical studies reveal that this cytokine, which we named IL-24, is a member of IL-10 family of cytokines, and it signals through two hetorodimeric receptors, whose expression is also upregulated by ras oncogenes. Thus, IL-24 and its receptors may represent a novel autocrine loop coordinately activated by ras oncogenes.
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Acknowledgments
We thank GenHunter Corporation for permission to adapt its protocol for RNAimage kit for differential display. The work was supported in part by grants awarded to P. Liang from the National Institutes of Health.
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© 2006 Humana Press Inc.
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Tan, Z., Wang, M., Liang, P. (2006). Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes. In: Liang, P., Meade, J.D., Pardee, A.B. (eds) Differential Display Methods and Protocols. Methods in Molecular Biology, vol 317. Humana Press. https://doi.org/10.1385/1-59259-968-0:207
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DOI: https://doi.org/10.1385/1-59259-968-0:207
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