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Development of Genetically Engineered Resistant Papaya for papaya ringspot virus in a Timely Manner

A Comprehensive and Successful Approach
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 354)

Abstract

Papaya orchards throughout most of the world are severely damaged by the destructive disease caused by the papaya ringspot virus (PRSV). PRSV-resistant papaya expressing the coat protein gene (CP) of PRSV have been used in Hawaii to control PRSV since 1998. This chapter presents the experimental steps involved in the development of transgenic papaya, including transgene construction, transformation, and analysis for virus resistance of the transformed papaya. We also describe the important factors that enabled deregulation, commercialization, and adoption of transgenic papaya to occur in Hawaii in a timely manner. Transfer of this technology to other countries with the similar goal and the development of transgenic papaya in other regions of the world also are described.

Key Words

Biolistic and Agrobacterium-mediated transformation coat protein gene papaya ringspot virus pathogen-derived resistance transgenic papaya 
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© Humana Press Inc. 2007

Authors and Affiliations

  1. 1.US Department of AgricultureUS Pacific Basin Agricultural Research CenterHilo

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