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Development of Genetically Engineered Resistant Papaya for papaya ringspot virus in a Timely Manner

A Comprehensive and Successful Approach

  • Protocol
Plant-Pathogen Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 354))

Abstract

Papaya orchards throughout most of the world are severely damaged by the destructive disease caused by the papaya ringspot virus (PRSV). PRSV-resistant papaya expressing the coat protein gene (CP) of PRSV have been used in Hawaii to control PRSV since 1998. This chapter presents the experimental steps involved in the development of transgenic papaya, including transgene construction, transformation, and analysis for virus resistance of the transformed papaya. We also describe the important factors that enabled deregulation, commercialization, and adoption of transgenic papaya to occur in Hawaii in a timely manner. Transfer of this technology to other countries with the similar goal and the development of transgenic papaya in other regions of the world also are described.

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Author information

Authors and Affiliations

  1. US Department of Agriculture, US Pacific Basin Agricultural Research Center, Hilo, HI

    Savarni Tripathi, Jon Suzuki & Dennis Gonsalves

Authors
  1. Savarni Tripathi
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  2. Jon Suzuki
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  3. Dennis Gonsalves
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Editors and Affiliations

  1. Department of Plant Pathology, University of California at Davis, Davis, CA

    Pamela C. Ronald

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© 2007 Humana Press Inc.

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Tripathi, S., Suzuki, J., Gonsalves, D. (2007). Development of Genetically Engineered Resistant Papaya for papaya ringspot virus in a Timely Manner. In: Ronald, P.C. (eds) Plant-Pathogen Interactions. Methods in Molecular Biology, vol 354. Humana Press. https://doi.org/10.1385/1-59259-966-4:197

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  • DOI: https://doi.org/10.1385/1-59259-966-4:197

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-448-7

  • Online ISBN: 978-1-59259-966-0

  • eBook Packages: Springer Protocols

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