Abstract
Virus-induced gene silencing (VIGS) is a powerful RNA-silencing based technology adapted for the study of host-gene function. VIGS functions through the expression of a host gene from a virus vector. Both the virus-encoded host sequence and the homologous host target messenger RNA are destroyed or made inactive through a host surveillance system. Here, we describe procedures for the use of a new virus vector for VIGS in monocotyledonous hosts and, in particular, in rice (Oryza sativa), a species for which no VIGS vector was previously available.
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Acknowlegments
The authors wish to thank Drs. Xian Zhi He and Justin Pita and Kim Ballard for helpful comments on the manuscript.
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Ding, X.S., Srinivasa Rao, C., Nelson, R.S. (2007). Analysis of Gene Function in Rice Through Virus-Induced Gene Silencing. In: Ronald, P.C. (eds) Plant-Pathogen Interactions. Methods in Molecular Biology, vol 354. Humana Press. https://doi.org/10.1385/1-59259-966-4:145
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DOI: https://doi.org/10.1385/1-59259-966-4:145
Publisher Name: Humana Press
Print ISBN: 978-1-58829-448-7
Online ISBN: 978-1-59259-966-0
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