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Chloroplast Transformation

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 318))

Abstract

In this chapter we briefly review the developmental history and current research status of chloroplast transformation and introduce the merits of chloroplast transformation as compared with the nuclear genome transformation. Furthermore, according to the chloroplast transformation achieved in oilseed rape (Brassica napus), we introduce the preparation of explants, transformation methods, system selection, identification methods of the transplastomic plants, and experimental results. The technical points, the bottleneck, and the further research directions of the chloroplast transformation are discussed in the notes.

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Acknowledgments

We thank Prof. Gao Xi-Wu for kindly providing the test insect P. xylostera, Dr. Pal Maliga for kindly providing aadA cassette, and Dr. Richard Wang for critically reviewing the manuscript. This project was supported by the MOST of China, NSFC, and CAS.

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Authors and Affiliations

  1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P.R. China

    Xiao-Mei Lu, Wei-Bo Yin & Zan-Min Hu

  2. Tomato Genetics Lab, Northeast Agriculture University, Harbin, P.R. China

    Xiao-Mei Lu

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  1. Xiao-Mei Lu
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  2. Wei-Bo Yin
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Editors and Affiliations

  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacion Cientifica de Yucatán, Yucatán, México

    Victor M. Loyola-Vargas  & Felipe Vázquez-Flota  & 

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© 2006 Humana Press Inc.

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Lu, XM., Yin, WB., Hu, ZM. (2006). Chloroplast Transformation. In: Loyola-Vargas, V.M., Vázquez-Flota, F. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology™, vol 318. Humana Press. https://doi.org/10.1385/1-59259-959-1:285

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  • DOI: https://doi.org/10.1385/1-59259-959-1:285

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-547-7

  • Online ISBN: 978-1-59259-959-2

  • eBook Packages: Springer Protocols

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