Abstract
Many dicotyledonous and also several monocotyledonous plant species are susceptible to Agrobacterium-mediated transformation. This current and well-established method has been used successfully with a large number of plant species to mediate gene transfer. This chapter describes an Agrobacterium-mediated transformation method of Petunia hybrida leaf discs, one of the first species that was routinely transformed using this method.
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References
Richie, S. W. and Hodeges, T. K. (1993) Cell culture and regeneration of transgenic plants, in Transgenic Plants, vol 1, (Kung, S. and Wu, R., eds), Academic Press, San Diego, pp. 147–178.
Jenes, B., Morre, H., Cao, J., Zhang, W., and Wu, R. (1993). Techniques for gene transfer, in Transgenic Plants, vol 1, (Kung, S. and Wu, R., eds), Academic Press, San Diego, pp. 125–146.
Bowen, B. A. (1993) Markers for gene transfer, in Transgenic Plants, vol 1, (Kung, S. and Wu, R., eds), Academic Press, San Diego, pp. 89–124.
Gerats, A. G. M., Huits, H., Vrijlandt, E., Mara, C., Souer, E., and Beld, M. (1990) Molecular characterization of a nonautonomous transposable element (dTph1) of petunia. Plant Cell 2, 1121–1128.
Horsch, R. B., Fry, J. E., Hoffman, N. L., Eichholtz, D., Rogers, S. C., and Fraley, R. T. (1985) A simple and general method for transferring genes into plants. Science 227, 1229–1231.
Hooykaas, P. J. J. (1989) Transformation of plant cells via Agrobacterium. Plant Mol. Biol. 13, 327–336.
Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 12, 8711–8721.
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15, 473–497.
Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Dellaporte, S. L., Wood, J., and Hicks, J. B. (1983) A plant DNA minipreparation: version II. Plant Mol. Biol. Rep. 1, 19–21.
Wang, H., Qi, M., and Cutler, A. J. (1993) A simple method of preparing plant samples for PCR. Nucleic Acids Res. 21, 4153–4154.
Hoekema, A., Hirsch, P. R., Hooykaas, P. J. J., and Schilperoort, R. A. (1983) A binary plant vector strategy based on separation of vir and T region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303, 179–180.
Lazo, G. R., Stein, P. A., and Ludwig, R. A. (1991) A DNA transformationcompetent Arabidopsis genomic library in Agrobacterium. Bio Technology 9, 963–967.
Gelvin, S. B. (2000) Agrobacterium and plant genes involved in T-DNA transfer and integration. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51, 223–256.
Li, W., Guo, G., and Zheng, G. (2000) Agrobacterium-mediated transformation: state of the art and future prospect. Chin. Science Bull. 45, 1537–1546.
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van der Meer, I.M. (2006). Agrobacterium-Mediated Transformation of Petunia Leaf Discs. In: Loyola-Vargas, V.M., Vázquez-Flota, F. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology™, vol 318. Humana Press. https://doi.org/10.1385/1-59259-959-1:265
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DOI: https://doi.org/10.1385/1-59259-959-1:265
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