Plant Cell Culture Protocols pp 265-272 | Cite as
Agrobacterium-Mediated Transformation of Petunia Leaf Discs
Protocol
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Abstract
Many dicotyledonous and also several monocotyledonous plant species are susceptible to Agrobacterium-mediated transformation. This current and well-established method has been used successfully with a large number of plant species to mediate gene transfer. This chapter describes an Agrobacterium-mediated transformation method of Petunia hybrida leaf discs, one of the first species that was routinely transformed using this method.
Key Words
Agrobacterium leaf disks Petunia hybrida protocol transformationReferences
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© Humana Press Inc. 2006