Summary
Ion-exchange chromatography techniques have been applied for the isolation of charged or ionizable natural products. The basis of the ion-exchange process is the reversible binding of either a cationic or an anionic molecule to an oppositely charged insoluble resin matrix by displacement of a counterion. Resins may be characterized by their matrix structure, and the “strength” of the exchanger is dependent upon the pK a of the ionized group. Choice of the type of resin and manipulation of pH conditions can be made to selectively bind ionized molecules. Elution of bound solute from the resin may be achieved by either adjustment of ion strength or changes in pH. The efficiency of the process may be enhanced by the use of gradient elution and inclusion of organic cosolvents. Application of the versatility of the technique is illustrated by a number of examples drawn from the literature.
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Durham, D.G. (2006). Isolation by Ion-Exchange Methods. In: Sarker, S.D., Latif, Z., Gray, A.I. (eds) Natural Products Isolation. Methods in Biotechnology, vol 20. Humana Press. https://doi.org/10.1385/1-59259-955-9:159
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DOI: https://doi.org/10.1385/1-59259-955-9:159
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