Abstract
The emergence of resistance to antifungal drugs in medically important fungi has become a significant problem in recent years. Probably the best-studied example is the development of resistance to the widely used antifungal agent fluconazole in the yeast Candida albicans. The availability of matched series of clinical isolates representing the same strain in which drug resistance developed over time has provided opportunities to detect cellular alterations that are correlated with drug resistance. We describe a method for DNA fingerprinting of C. albicans isolates based on Southern hybridization of genomic DNA with the C. albicans-specific repetitive DNA element CARE-2. Molecular typing with CARE-2 permits highly reliable discrimination of unrelated strains to ascertain that serial isolates recovered from individual patients indeed represent the same C. albicans strain.
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References
White, T. C., Marr, K. A., and Bowden, R. A. (1998) Clinical, cellular, and molecular factors that contribute to antifungal drug resistance. Clin. Microbiol. Rev. 11, 382–402.
Soll, D. R. (2000) The ins and outs of DNA fingerprinting the infectious fungi. Clin. Microbiol. Rev. 13, 332–370.
Lasker, B. A., Page, L. S., Lott, T. J., and Kobayashi, G. S. (1992). Isolation, characterization, and sequencing of Candida albicans repetitive element 2. Gene 116, 51–57.
Lockhart, S. R., Reed, B. D., Pierson, C. L., and Soll, D. R. (1996). Most frequent scenario for recurrent Candida vaginitis is strain maintenance with “substrain shuffling”: demonstration by sequential DNA fingerprinting with probes Ca3, C1, and CARE2. J. Clin. Microbiol. 34, 767–777.
Acknowledgments
We thank Katherine Barker for critical reading of the manuscript. Work in our laboratories is supported by the Deutsche Forschungsgemeinschaft, the Bundesministerium für Bildung und Forschung, the Bayerische Forschungsgemeinschaft, the European Union, and the National Institutes of Health.
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© 2005 Humana Press Inc.
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Morschhäuser, J., Staib, P., Köhler, G. (2005). CARE-2 Fingerprinting of Candida albicans Isolates. In: Ernst, E.J., Rogers, P.D. (eds) Antifungal Agents. Methods in Molecular Medicine™, vol 118. Humana Press. https://doi.org/10.1385/1-59259-943-5:027
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DOI: https://doi.org/10.1385/1-59259-943-5:027
Publisher Name: Humana Press
Print ISBN: 978-1-58829-277-3
Online ISBN: 978-1-59259-943-1
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