Abstract
Culture and transport methods are described that allow chromosomes to be obtained from lymph node biopsies with a high success rate, even when the biopsy is taken at a distant site from the cytogenetic laboratory. Optimal banding techniques for the identification of these chromosomes are given, along with hints on how to correct any problems encountered on initial staining. We also describe how to prepare this material for fluorescence in situ hybridization techniques, both for further delineation of complex chromosomal rearrangements and for interphase analysis in cases in which conventional cytogenetics has not been successful.
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References
Heim, S. and Mitelman, F. (1995) Cancer Cytogenetics. Wiley Liss, New York.
Webber, L. M. and Garson, O. M. (1983) Fluorodeoxyuridine synchronization of bone marrow cultures. Cancer Genet. Cytogenet. 8, 123–132.
Gahrton, G., Robert, K.-H., Friberg, K., Zech, L., and Bird, A. (1980) Nonrandom chromosomal aberrations in chronic lymphocytic leukemia revealed by polyclonal B-cell mitogen stimulation. Blood 56, 640–647.
Schrock, E., du Manoir, S., Veldman, T., Schoell, B., Weinberg, J., Ferguson-Smith, M. A., et al. (1996) Multicolor spectral karyotyping of human chromosomes. Science 273, 494–497.
Speicher, M. R., Gwyn Ballard, S., and Ward, D. C. (1996) Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nat. Genet. 12, 368–375.
Sanchez, O., Escobar, J. J., and Yunis, J. J. (1973) A simple G banding technique. Lancet ii, 279.
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© 2005 Humana Press Inc.
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Ross, F.M., Harrison, C.J. (2005). Karyotyping Lymph Node Biopsies in Non-Hodgkin’s Lymphoma. In: Illidge, T., Johnson, P.W.M. (eds) Lymphoma. Methods in Molecular Medicine™, vol 115. Humana Press. https://doi.org/10.1385/1-59259-936-2:093
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DOI: https://doi.org/10.1385/1-59259-936-2:093
Publisher Name: Humana Press
Print ISBN: 978-1-58829-159-2
Online ISBN: 978-1-59259-936-3
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