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Immunocytochemical Localization of Receptors Using Light and Confocal Microscopy With Application to the Phenotypic Characterization of Knock-Out Mice

  • Anthony P. Davenport
  • Rhoda E. Kuc
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Part of the Methods in Molecular Biology™ book series (MIMB, volume 306)

Abstract

This chapter describes the immunocytochemical (ICC) localization of receptors in tissue sections and cells growing in culture. The protocol is illustrated using examples of how site-directed polyclonal antisera can be used to distinguish between subtypes of G protein-coupled receptors (GPCRs; endothelin ETA and ETB), (1) in sections of human tissue or whole-body sections of mice, as well as mapping previously designated orphan receptors (oGPCR) recently paired with their cognate ligand illustrated by visualizing receptors for the peptide ghrelin (2). The methods are applicable to other receptor families.

Keywords

Alexa Fluor Primary Antiserum Secondary Antiserum Swine Serum Nonspecific Background Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Anthony P. Davenport
    • 1
  • Rhoda E. Kuc
    • 1
  1. 1.Clinical Pharmacology UnitUniversity of Cambridge, Centre for Clinical Investigation, Addenbrooke’s HospitalCambridgeUK

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