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Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels

  • David A. Walsh
  • John Wharton
Protocol
  • 593 Downloads
Part of the Methods in Molecular Biology™ book series (MIMB, volume 306)

Abstract

Autoradiographic detection of ligand binding to tissue sections has been used to localize, quantify, and characterize a diverse range of sites. Enzymes have been studied via selective inhibitors, ion channels using naturally occurring toxins, and second messenger systems using inositol polyphosphates. Ligand binding complements immunohistochemistry (see  Chapter 8) and in situ hybridization (see  Chapter 4) by permitting pharmacological characterization and quantification of active sites. Localization, affinity, and specificity of binding sites for ligands (see  Chapter 5) can be correlated with functional studies performed with the same pharmacological agent. Bioactive ligands are often identified before their targets have been fully characterized, and radiolabeled ligands may become available before molecular and immunological reagents have been developed. A pharmacologically active agent may be synthesized before the endogenous ligand for its binding site has been identified, and autoradiographic methods may help elucidate the site of action of such agents.

Keywords

Nitric Oxide Synthases Vanilloid Receptor Wire Loop Inositol Polyphosphate Paper Clip 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • David A. Walsh
    • 1
  • John Wharton
    • 2
  1. 1.Academic RheumatologyUniversity of Nottingham, City HospitalNottinghamUK
  2. 2.Section on Experimental Medicine and ToxicologyImperial College LondonLondonUK

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