Quantitative Analysis of Orphan G Protein-Coupled Receptor mRNAs by TaqMan® Real-Time PCR
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Historically, the G protein-coupled receptor (GPCR) protein family has proven to be an extremely tractable target class (1). It is estimated that approximately one-half of all drugs currently marketed exert their actions, either directly or indirectly, via GPCRs (2). Given the potential commercial opportunities emanating from the identification of small molecule modulators of “novel” GPCRs (currently, GPCRs generate in excess of $25 billion per year in worldwide sales revenue ), it is not surprising that it is with great enthusiasm that both the pharmaceutical industry and academia move toward identifying novel members of this protein class.
KeywordsPolymerase Chain Reaction GPR4 Expression RNeasy Column TaqMan Polymerase Chain Reaction Polymerase Chain Reaction Process
- 15.Sambrook, J., Fritsch, E.F., and Maniatis, T. (eds.) (1989) Molecular Cloning: ALaboratory Manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, NY.Google Scholar