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Quantitative Analysis of Orphan G Protein-Coupled Receptor mRNAs by TaqMan® Real-Time PCR

G2A and GPR4 Lysophospholipid Receptor Expression in Leukocytes and in a Rat Myocardial Infarction-Heart Failure Model
  • Stephen A. Douglas
  • Zhaohui Ao
  • Douglas G. Johns
  • Kristeen Maniscalco
  • Robert N. Willette
  • Lea Sarov-Blat
  • John P. Cogswell
  • Sheila Seepersaud
  • Paul Murdock
  • Klaudia M. Steplewski
  • Lisa Patel
Protocol
  • 610 Downloads
Part of the Methods in Molecular Biology™ book series (MIMB, volume 306)

Abstract

Historically, the G protein-coupled receptor (GPCR) protein family has proven to be an extremely tractable target class (1). It is estimated that approximately one-half of all drugs currently marketed exert their actions, either directly or indirectly, via GPCRs (2). Given the potential commercial opportunities emanating from the identification of small molecule modulators of “novel” GPCRs (currently, GPCRs generate in excess of $25 billion per year in worldwide sales revenue [3]), it is not surprising that it is with great enthusiasm that both the pharmaceutical industry and academia move toward identifying novel members of this protein class.

Keywords

Polymerase Chain Reaction GPR4 Expression RNeasy Column TaqMan Polymerase Chain Reaction Polymerase Chain Reaction Process 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Stephen A. Douglas
  • Zhaohui Ao
  • Douglas G. Johns
  • Kristeen Maniscalco
  • Robert N. Willette
  • Lea Sarov-Blat
  • John P. Cogswell
  • Sheila Seepersaud
  • Paul Murdock
  • Klaudia M. Steplewski
  • Lisa Patel

There are no affiliations available

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