Abstract
Gel electrophoretic analysis of proteins is a well-established method for separating, quantifying, identifying, comparing, and characterizing proteins. This form of analysis consists of a diverse class of methods, with new approaches and improvements being frequently added, including computer software dedicated to the analysis of protein gels. Protein electrophoresis involves four principal steps: protein harvesting, in-gel separation, visualization of separated proteins, and data collection and analysis. Proteins are extremely heterogeneous, and no single method will recover all cellular proteins equally effectively. In its native state, a protein may be soluble, membrane-, or organelle-bound and may show posttranslational and pH-dependent modifications.
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O’Callaghan, K.J., Byrne, L.J., Tuite, M.F. (2005). Extraction and Denaturing Gel Electrophoretic Methodology for the Analysis of Yeast Proteins. In: Smales, C.M., James, D.C. (eds) Therapeutic Proteins. Methods in Molecular Biology™, vol 308. Humana Press. https://doi.org/10.1385/1-59259-922-2:357
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DOI: https://doi.org/10.1385/1-59259-922-2:357
Publisher Name: Humana Press
Print ISBN: 978-1-58829-390-9
Online ISBN: 978-1-59259-922-6
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