Abstract
Multiple myeloma is a B-cell neoplasm characterized by the monoclonal proliferation of plasma cells in the bone marrow, the development of osteolytic lesions, and the induction of angiogenesis. These different processes require three-dimensional interactions, with both humoral and cellular contacts. The 5TMM models are suitable to study these interactions. These are murine models that originate from spontaneously developed myeloma in elderly mice. They are propagated by in vivo transfer of the myeloma cells into young syngeneic mice. We report methods involving the maintenance of the 5T2MM model and the quantification of tumor burden (by determining serum paraprotein concentration and plasmacytosis), assessment of bone lesions, and quantification of angiogenesis. The combination of these different techniques in these models not only helps in unraveling basic biological processes but also in the testing of potentially new therapeutic targets.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Radl J. (1990) Age-related monoclonal gammapathies: clinical lessons from the aging C57BL mouse. Immunol. Today 11, 234–236.
Radl J., De Glopper E. D., Schuit H. R., and Zurcher C. (1979) Idiopathic paraproteinemia. II. Transplantation of the paraprotein-producing clone from old to young C57BL/KaLwRij mice. J. Immunol. 122, 609–613.
Vanderkerken K., Asosingh K., Croucher P., and Van Camp B. (2003) Multiple myeloma biology: lessons from the 5TMM models. Immunol. Rev. 194, 196–206.
Asosingh K., Radl J., Van Riet I., Van Camp B., and Vanderkerken K. (2000) The 5TMM series: a useful in vivo mouse model of human multiple myeloma. Hematol. J. 1, 351–356.
Vanderkerken K., Goes E., De Raeve H., Radl J., and Van Camp B. (1996) Follow-up of bone lesions in an experimental multiple myeloma mouse model: description using X-ray dedicated for mammography. Br. J. Cancer 73, 1463–1465.
Vanderkerken K., De Raeve H., Goes E., et al. (1997) Organ involvement and phenotypic adhesion profile of 5T2 and 5T33 myeloma cells in the C57BL/ KaLwRij mouse. Br. J. Cancer 76, 451–460.
Croucher P. I., Shipman C., Lippitt J., et al. (2001) Osteoprotegerin inhibits the development of osteolytic bone disease in multiple myeloma. Blood 98, 3534–3540.
Van Valckenborgh E., De Raeve H., Devy L., et al. (2002) Murine 5T multiple myeloma cells induce angiogenesis in vitro and in vivo. Br. J. Cancer 86, 796–802.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
Vanderkerken, K. et al. (2005). The 5T2MM Murine Model of Multiple Myeloma. In: Brown, R.D., Ho, P.J. (eds) Multiple Myeloma. Methods in Molecular Medicine™, vol 113. Humana Press. https://doi.org/10.1385/1-59259-916-8:191
Download citation
DOI: https://doi.org/10.1385/1-59259-916-8:191
Publisher Name: Humana Press
Print ISBN: 978-1-58829-392-3
Online ISBN: 978-1-59259-916-5
eBook Packages: Springer Protocols