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Ligand Binding With Stopped-Flow Rapid Mixing

  • Mark S. Hargrove
Part of the Methods in Molecular Biology™ book series (MIMB, volume 305)

Abstract

Stopped-flow rapid mixing is a common, direct technique for the study of ligand-binding reactions. In this method, protein and ligand are mixed together at relatively high velocities directly into an observation chamber, so that time courses for reactions occurring on time scales as short as a few milliseconds can be measured. This chapter presents an introduction to this technique, including a discussion of experimental and technical issues that must be addressed when designing stopped-flow experiments. Simple experiments for measuring reaction dead time and flushing volume are also described along with the details of several common reaction schemes and methods for data analysis.

Key Words

Stopped-flow mixing kinetics ligand binding 

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Mark S. Hargrove
    • 1
  1. 1.Department of Biochemistry, Biophysics and Molecular BiologyIowa State UniversityAmes

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