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Direct Optical Detection of Protein-Ligand Interactions

  • Frank Gesellchen
  • Bastian Zimmermann
  • Friedrich W. Herberg
Part of the Methods in Molecular Biology™ book series (MIMB, volume 305)

Abstract

Direct optical detection provides an excellent means to investigate interactions of molecules in biological systems. The dynamic equilibria inherent to these systems can be described in greater detail by recording the kinetics of a biomolecular interaction. Optical biosensors allow direct detection of interaction patterns without the need for labeling. An overview covering several commercially available biosensors is given, with a focus on instruments based on surface plasmon resonance (SPR) and reflectometric interference spectroscopy (RIFS). Potential assay formats and experimental design, appropriate controls, and calibration procedures, especially when handling low molecular weight substances, are discussed. The single steps of an interaction analysis combined with practical tips for evaluation, data processing, and interpretation of kinetic data are described in detail. In a practical example, a step-by-step procedure for the analysis of a low molecular weight compound interaction with serum protein, determined on a commercial SPR sensor, is presented.

Key Words

Optical biosensors surface plasmon resonance reflectometric interference spectroscopy biomolecular interaction analysis kinetics low molecular weight ligands 

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Frank Gesellchen
    • 1
  • Bastian Zimmermann
    • 2
  • Friedrich W. Herberg
    • 1
  1. 1.Department of BiochemistryUniversity of KasselKasselGermany
  2. 2.Biaffin GmbH & Co KGKasselGermany

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