Summary
HIV-1 can be isolated from peripheral blood mononuclear cells and is easily propagated on primary cells in vitro. Here we describe the method for bulk isolation of the HIV-1 quasispecies and a limiting dilution virus isolation protocol by which single coexisting clones can be obtained. In addition, methods for propagation and titration of HIV-1 are provided.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Popovic, M., Sarngadharan, M. G., Read, E., and Gallo, R. C. (1984) Detection, isolation and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224, 497.
Sawyer, L. S. W., Wrin, M. T., Crawford-Mikza, L., Potts, B., Wu, Y., Weber, P. A., et al. (1994) Neutralization sensitivity of human immunodeficiency virus type 1 is determined in part by the cell in which the virus is propagated. J. Virol. 68, 1342–1349.
De Roda Husman, A. M., Koot, M., Cornelissen, M., Brouwer, M., Broersen, S. M., Bakker, M., et al. (1997) Association between CCR5 genotype and the clinical course of HIV-1 infection. Ann. Intern. Med. 127, 882–890.
Schuitemaker, H., Koot, M., Kootstra, N. A., Dercksen, M. W., De Goede, R. E. Y., Van Steenwijk, R. P., et al. (1992) Biological phenotype of human immunodeficiency virus type 1 clones at different stages of infection: progression of disease is associated with a shift from monocytotropic to T-cell-tropic virus populations. J. Virol. 66, 1354–1360.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
Schuitemaker, H., Kootstra, N.A. (2005). Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals. In: Zhu, T. (eds) Human Retrovirus Protocols. Methods in Molecular Biology™, vol 304. Humana Press. https://doi.org/10.1385/1-59259-907-9:017
Download citation
DOI: https://doi.org/10.1385/1-59259-907-9:017
Publisher Name: Humana Press
Print ISBN: 978-1-58829-495-1
Online ISBN: 978-1-59259-907-3
eBook Packages: Springer Protocols