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Use of Proteomics to Study Chemosensitivity

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Chemosensitivity: Volume II

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 111))

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Abstract

Chemoresistance remains an unresolved problem in clinical oncology. Therefore it is impor tant to identify molecular factors that lead to an understanding of the mechanisms of drug resis tance in cancer cells. On the protein-expression level,this can be done using proteomics,which has become the focus of significant interest and research over the past decade.

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References

  1. Bernardini, S., Bellincampi, L., Ballerini, S., et al. (2002) Role of GST P1-1 in mediating the effect of etoposide on human neuroblastoma cell line Sh-Sy5y. J. Cell Biochem. 86, 340–347.

    Article  CAS  PubMed  Google Scholar 

  2. Rabilloud, T. (1998) Use of thiourea to increase the solubility of membrane pro teins in two-dimensional electrophoresis. Electrophoresis 19, 758–760.

    Article  CAS  PubMed  Google Scholar 

  3. Cheung, C. K., Mak, Y. T., and Swaminathan, R. (1987) Automated trichloroacetic acid precipitation method for urine total protein. Ann. Clin. Biochem. 24, 140–144.

    Article  CAS  PubMed  Google Scholar 

  4. O/rsFarrell, P. H. (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021.

    Google Scholar 

  5. Klose, J. (1975) Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals. Humangenetik 26, 231–243.

    CAS  PubMed  Google Scholar 

  6. Scheele, G. A. (1975) Two-dimensional gel analysis of soluble proteins. Charaterization of guinea pig exocrine pancreatic proteins. J. Biol. Chem. 250, 5375–5385.

    CAS  PubMed  Google Scholar 

  7. Bjellqvist, B., Ek, K., Righetti, P. G., et al. (1982) Isoelectric focusing in immo bilized pH gradients: principle,methodology and some applications. J. Biochem. Biophys. Meth. 6, 317–339.

    Article  CAS  PubMed  Google Scholar 

  8. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.

    Article  CAS  PubMed  Google Scholar 

  9. Heukeshoven, J. and Dernick, R. (1995) Effective blotting of ultrathin polyacry lamide gels anchored to a solid matrix. Electrophoresis 6, 103–112.

    Article  Google Scholar 

  10. Klose, J. and Kobalz, U. (1995) Two-dimensional electrophoresis of proteins: an updated protocol and implications for a functional analysis of the genome. Elec trophoresis 16, 1034–1059.

    Article  CAS  Google Scholar 

  11. Neuhoff, V., Arold, N., Taube, D., and Ehrhardt, W. (1988) Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear back ground at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9, 255–262.

    Article  CAS  PubMed  Google Scholar 

  12. Dreger, M., Bengtsson, L., Schoneberg, T., Otto, H., and Hucho, F. (2001) Nuclear envelope proteomics: novel integral membrane proteins of the inner nuclear membrane. Proc. Natl. Acad. Sci USA 98, 11,943–11,948.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Schirmer, E. C. and Gerace, L. (2002) Organellar proteomics: the prizes and pitfalls of opening the nuclear envelope. Genome Biol. 3,REVIEWS 1008.

    Article  Google Scholar 

  14. Dreger, M. (2003) Proteome analysis at the level of subcellular structures. Eur. J. Biochem. 270, 589–599.

    Article  CAS  PubMed  Google Scholar 

  15. Shevchenko, A., Wilm, M., Vorm, O., and Mann, M. (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68, 850–858.

    Article  CAS  PubMed  Google Scholar 

  16. Sinha, P., Poland, J., Schnolzer, M., and Rabilloud, T. (2001) A new silver staining apparatus and procedure for matrix-assisted laser desorption/ionization-time of flight analysis of proteins after two-dimensional electrophoresis. Proteomics 1, 835–840.

    Article  CAS  PubMed  Google Scholar 

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© 2005 Humana Press Inc.

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Poland, J., Wandschneider, S., Urbani, A., Bernardini, S., Federici, G., Sinha, P. (2005). Use of Proteomics to Study Chemosensitivity. In: Blumenthal, R.D. (eds) Chemosensitivity: Volume II. Methods in Molecular Medicine™, vol 111. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-889-7:267

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  • DOI: https://doi.org/10.1385/1-59259-889-7:267

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-586-6

  • Online ISBN: 978-1-59259-889-2

  • eBook Packages: Springer Protocols

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