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Competitive Enzyme-Linked Immunosorbent Assay

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Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 295))

Abstract

Competitive enzyme-linked immunosorbent assay provides an alternative to dual-antibody sandwich enzyme-linked immunosorbent assay and is widely used for the measurement of substances in biological liquids.

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References

  1. Kemeny, D. M. and Challacombe, S. J. (1988) ELISA and Other Solid Phase Imunoassays. Theory and Practical Aspects. John Wiley and Sons, Chicester, UK.

    Google Scholar 

  2. Kemeny, D. M (1991) A Practical Guide to ELISA. Permagon Press, Oxford, UK.

    Google Scholar 

  3. Esser, P. (1990) Detergent in Polystyrene ELISA. Nunc Bulletin No. 8, second edition 1997, 1–5.

    Google Scholar 

  4. Jenny, R. J., Messier, T. L., Ouellette, L. A., and Church, W. R. (1993) Immunochemical techniques for studying coagulation proteins. Methods Enzymol. 222, 400–416.

    Article  PubMed  CAS  Google Scholar 

  5. Bronstein, I., Voyta, J. C., Thorpe, G. H., Kricka, L. J., and Armstrong, G. (1989) Chemiluminescent assay of alkaline phosphatase applied in an ultrasensative enzyme immunoassay of thyrotropin. Clin. Chem. 35, 1441–1446.

    PubMed  CAS  Google Scholar 

  6. Cook, D. B. and Self, C. H. (1993) Determination of one-thousandth of an attomole (1zeptomole) of alkaline phosphatase: application in an immunoassay of proinsulin. Clin. Chem. 39, 965–971.

    PubMed  CAS  Google Scholar 

  7. Self, C. H. (1985) Enzyme amplification-a general method applied to provide an immunoassisted assay for placental alkaline phosphatase. J. Immunological Methods 76, 389–393.

    Article  CAS  Google Scholar 

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© 2005 Humana Press Inc., Totowa, NJ

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Jordan, W. (2005). Competitive Enzyme-Linked Immunosorbent Assay. In: Burns, R. (eds) Immunochemical Protocols. Methods In Molecular Biology™, vol 295. Humana Press. https://doi.org/10.1385/1-59259-873-0:215

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  • DOI: https://doi.org/10.1385/1-59259-873-0:215

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-274-2

  • Online ISBN: 978-1-59259-873-1

  • eBook Packages: Springer Protocols

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