Immunochemical Protocols pp 127-130 | Cite as
Conjugation of Antibodies to Horseradish Peroxidase
Protocol
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Abstract
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups are allowed to react with amino groups in the IgG antibody. The Schiff bases formed are reduced and the conjugate is purified by gel filtration.
Key Words
Antibody horseradish peroxidase conjugation labeling periodate oxidationReferences
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© Humana Press Inc., Totowa, NJ 2005