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Medical Biomethods Handbook pp 327–345Cite as

Quantitative PCR

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Abstract

Commonly used methods to quantify RNA and DNA include Northern and Southern blotting, RNase protection assays, and in situ hybridization (see Chapter 29). Because these methods analyze nonamplified RNA or DNA, they are of low sensitivity and require relatively large amounts of nucleic acid. Another method, thousands of times more sensitive than these traditional techniques, combines reverse transcription (RT) and the polymerase chain reaction (PCR). Although RT-PCR is an exquisitely sensitive and specific technique, obtaining quantitative data presents a difficult challenge (1–4).

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  1. Centre for Reproduction, Endocrinology, and Diabetes, School of Biomedical Sciences, King’s College London, London, UK

    David Sugden

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  1. University of Hertfordshire, Hatfield, UK

    John M. Walker  & Ralph Rapley  & 

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Sugden, D. (2005). Quantitative PCR. In: Walker, J.M., Rapley, R. (eds) Medical Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-870-6:327

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  • DOI: https://doi.org/10.1385/1-59259-870-6:327

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