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Influence of Radiation Protocols on Graft-vs-Host Disease Incidence After Bone-Marrow Transplantation in Experimental Models

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Adoptive Immunotherapy: Methods and Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 109))

Abstract

Bone-marrow transplantation is an approved curative treatment for many hemato- and oncologic diseases. Nevertheless, the severe acute clinical course of graft-vs-host disease (GVHD) after allogeneic bone-marrow transplantation is frequently fatal, and is to date not curable. Acute GVHD must, therefore, be prevented from the start of the bone-marrow transplantation by immunosuppressive medication, causing sometimes serious side effects. Therefore, new preventive strategies are tested, starting with animal experiments. Often mice are chosen for this kind of trial, and the clinical protocol of bone-marrow transplantation is transferred into the experimental settings. The first step to induce an acute GVHD is whole-body irradiation of the recipients. Several methods are available for this purpose: the most common is a 60cobalt source (γ-irradiation); less common are a 137cesium source (γ-irradiation) and a linear (particle) accelerator (photons). Differences between these radiation techniques can occur and can unexpectedly interfere with the results of the experiments. In this chapter, the materials and methods for bone-marrow transplantation in mice, with particular emphasis on the different radiation techniques, are explained; furthermore, the advantages and disadvantages in regard to the underlying physical principles will be discussed.

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Author information

Authors and Affiliations

  1. Department of Radiooncology, Hannover Medical School, Hannover, Germany

    Sebastian Schwarte

  2. Department of Abdominal and Transplant Surgery, Hannover Medical School, Hannover, Germany

    Matthias W. Hoffmann

Authors
  1. Sebastian Schwarte
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  2. Matthias W. Hoffmann
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Editor information

Editors and Affiliations

  1. Research Department, Kantonsspital St. Gallen, Switzerland

    Burkhard Ludewig

  2. Department of Abdominal and Transplant Surgery, Hannover Medical School, Hannover, Germany

    Matthias W. Hoffmann

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© 2005 Humana Press Inc.

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Schwarte, S., Hoffmann, M.W. (2005). Influence of Radiation Protocols on Graft-vs-Host Disease Incidence After Bone-Marrow Transplantation in Experimental Models. In: Ludewig, B., Hoffmann, M.W. (eds) Adoptive Immunotherapy: Methods and Protocols. Methods in Molecular Medicine™, vol 109. Humana Press. https://doi.org/10.1385/1-59259-862-5:445

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  • DOI: https://doi.org/10.1385/1-59259-862-5:445

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-406-7

  • Online ISBN: 978-1-59259-862-5

  • eBook Packages: Springer Protocols

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