Skip to main content

Primary Culture of Human Antral Endocrine and Epithelial Cells

  • Protocol
Book cover Human Cell Culture Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 107))

  • 2493 Accesses

Abstract

The mucosal endocrine cells in the antrum are found as individual elements interspersed among the surrounding epithelial cells, the majority of these being the gastric mucous cells. To establish the factors regulating either endocrine or mucous cell function, the cells have to be separated not only from nonepithe-lial cells, but also from circulating and neuronal elements within the stomach.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Soll, A., Amirian, D., Park, J., Elashoff, J., and Yamada, T. (1985) Cholecystokinin potently releases somatostatin from canine fundic mucosal cells in short-term culture. Am. J. Physiol. 248, G659–G573.

    Google Scholar 

  2. Squires, P. E., Meloche, R. M., and Buchan, A. M. J. (1999) Bombesin-evoked gastrin release and calcium-signaling in human antral G-cells in culture. Am. J. Physiol. 276, G227–G237.

    PubMed  CAS  Google Scholar 

  3. Buchan, A. M. J., Curtis, S. B., and Meloche, R. M. (1990) Release of somato-statin-immunoreactivity from human antral D cells in culture. Gastroenterology 99, 690–696.

    PubMed  CAS  Google Scholar 

  4. Richter-Dahlfors, A., Meloche, R. M., Finlay, B. B., and Buchan, A. M. J. (1998) Helicobacter pylori infected human antral primary cell cultures: effect on gastrin cell function. Am. J. Physiol. 275, G393–G401.

    PubMed  CAS  Google Scholar 

  5. Buchan, A. M. J. (1991) Effect of sympathomimetics on gastrin secretion from antral cells in culture. J. Clin. Invest. 87, 1382–1386.

    Article  PubMed  CAS  Google Scholar 

  6. Buchan, A. M. J., MacLeod, M. D., Meloche, R. M., and Kwok, Y. N. (1992) Mus-carinic regulation of somatostatin release from primary cultures of human antral epithelial cells. Pharmacology 44, 33–40.

    Article  PubMed  CAS  Google Scholar 

  7. Buchan, A. M. J., Meloche, R. M., Kwok, Y. N., and Kofod, H. (1993) Effect of CCK and secretin on somatostatin release from cultured antral cells. Gastroenterology 104, 1414–1419.

    PubMed  CAS  Google Scholar 

  8. Backhed, F., Rokbi, B., Tortensson, E., et al. (2003) Gastric mucosal recognition of Helicobacter pylori is independent of Toll-like receptor 4. J. Inf. Dis. 203, 829–836.

    Article  Google Scholar 

  9. Guarnieri, M., Krell, L. S., McKhann, G. M., Pasternak, G. W., and Yamamura, H.I. (1975) The effects of cell isolation techniques on neuronal membrane receptors. Brain Res. 93, 337–342.

    Article  PubMed  CAS  Google Scholar 

  10. Meinstrich, M. L. (1983) Experimental factors involved in separation by centrifugal elutriation, in Cell Separation Methods and Selected Application, vol. 2 (Pretlow, T. G. and Pretlow, T. P., eds.), Academic, New York, pp. 33–61.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Humana Press Inc.

About this protocol

Cite this protocol

Curtis, S.B., Buchan, A.M.J. (2005). Primary Culture of Human Antral Endocrine and Epithelial Cells. In: Picot, J. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine™, vol 107. Humana Press. https://doi.org/10.1385/1-59259-861-7:313

Download citation

  • DOI: https://doi.org/10.1385/1-59259-861-7:313

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-222-3

  • Online ISBN: 978-1-59259-861-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics