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Nanostructured Systems for Biological Materials

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Protein Nanotechnology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 300))

Summary

The sol-gel process is a chemical technique for immobilizing biomolecules in an inorganic, transparent matrix. The dopant biomolecules reside in an interconnected mesoporous network and become part of the nanostructured architecture of the entire material. In this chapter, we review the sol-gel immobilization approach and discuss how it leads to the stabilization of a number of proteins against aggressive chemical and thermal environments. We also review the sensor applications of this material that result from having analyte molecules diffuse through the matrix and reach the immobilized biomolecule.

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Acknowledgments

We greatly appreciate the contributions of Dr. Dorothy Nguyen, Dr. Jenna Rickus, Jing C. Zhou, James R. Lim, Maria H. Chuang, and Pauline Chang to the work described in this review. We also gratefully acknowledge support for this research from NSF (DMR 0103952 and DMR 0099862) and NASA (NAG9-1252). This work was also partially supported by the Center for Cell Mimetic Space Exploration, a NASA University Research, Engineering and Technology Institute, through award no. NCC 2-1364.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, University of California at Los Angeles, Los Angeles, CA

    Esther H. Lan & Bruce Dunn

  2. Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA

    Jeffrey I. Zink

Authors
  1. Esther H. Lan
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  2. Bruce Dunn
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  3. Jeffrey I. Zink
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Editor information

Editors and Affiliations

  1. Center for Advanced Biomedical Photonics, Oak Ridge National Laboratory, Oak Ridge, TN

    Tuan Vo-Dinh (Director) (Director)

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© 2005 Humana Press Inc., Totowa, NJ

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Lan, E.H., Dunn, B., Zink, J.I. (2005). Nanostructured Systems for Biological Materials. In: Vo-Dinh, T. (eds) Protein Nanotechnology. Methods in Molecular Biology™, vol 300. Humana Press. https://doi.org/10.1385/1-59259-858-7:053

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  • DOI: https://doi.org/10.1385/1-59259-858-7:053

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-310-7

  • Online ISBN: 978-1-59259-858-8

  • eBook Packages: Springer Protocols

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