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The Xenopus Cell Cycle

An Overview

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Book cover Cell Cycle Control

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 296))

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Abstract

Oocytes, eggs, and embryos from the frog Xenopus laevis have been an important model system for studying cell cycle regulation for several decades. First, progression through meiosis in the oocyte has been extensively investigated. Oocyte maturation has been shown to involve complex networks of signal transduction pathways, culminating in the cyclic activation and inactivation of maturation promoting factor (MPF), which is composed of cyclin B and cdc2. After fertilization, the early embryo undergoes rapid simplified cell cycles, which have been recapitulated in cell-free extracts of Xenopus eggs. Experimental manipulation of these extracts has given a wealth of biochemical information about the cell cycle, particularly concerning DNA replication and mitosis. Finally, cells of older embryos adopt a more somatictype cell cycle and have been used to study the balance between cell cycle and differentiation during development.

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Authors
  1. Anna Philpott
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  2. P. Renee Yew
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Editor information

Editors and Affiliations

  1. MRC Radiation and Genome Stability Unit, Harwell, Didcot, UK

    Tim Humphrey

  2. School of Pharmacy, University of Reading Whiteknights, Reading, UK

    Gavin Brooks

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© 2005 Humana Press Inc.

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Philpott, A., Renee Yew, P. (2005). The Xenopus Cell Cycle. In: Humphrey, T., Brooks, G. (eds) Cell Cycle Control. Methods in Molecular Biology™, vol 296. Humana Press. https://doi.org/10.1385/1-59259-857-9:095

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  • DOI: https://doi.org/10.1385/1-59259-857-9:095

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-144-8

  • Online ISBN: 978-1-59259-857-1

  • eBook Packages: Springer Protocols

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