DNA Viruses pp 299-315 | Cite as
Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection
Protocol
- 3 Citations
- 1.2k Downloads
Abstract
This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons.
Key Words
Cultured neuron herpesvirus immunofluorescence infection laminin nerve growth factor neuron ornithine PC12 pheochromocytoma pseudorabies virus superior cervical ganglia sympathetic neuronsReferences
- 1.Banker, G. and Goslin, K. (eds.) (1998) Culturing Nerve Cells, 2nd ed. MIT Press, Cambridge, MA.Google Scholar
- 2.Fedoroff, S. and Richardson, A. (eds.) (2001) Protocols for Neural Cell Culture, 3rd ed. Humana, Totowa, NJ.CrossRefGoogle Scholar
- 3.Tomishima, M. J., Smith, G. A., and Enquist, L. W. (2001) Sorting and transport of alpha herpesviruses in axons. Traffic 2, 429–436.PubMedCrossRefGoogle Scholar
- 4.Tischler, A. S. and Greene, L. A. (1975) Nerve growth factor-induced process formation by cultured rat pheochromocytoma cells. Nature 258, 341–342.PubMedCrossRefGoogle Scholar
- 5.Greene, L. A., Burstein, D. E., and Black, M. M. (1982) The role of transcriptiondependent priming in nerve growth factor promoted neurite outgrowth. Dev. Biol. 91, 305–316.PubMedCrossRefGoogle Scholar
- 6.Greene, L. A., Farinelli, S. E., Cunningham, M. E., and Park, D. S. (1998) Culture and experimental use of the PC12 rat pheochromocytoma cell line, in Culturing Nerve Cells, 2nd ed. (Banker, G. and Goslin, K., eds.), MIT Press, Cambridge, MA, pp. 161–188.Google Scholar
- 7.Mobley, W. C, Schenker, A., and Shooter, E. M. (1976) Characterization and isolation of proteolytically modified nerve growth factor. Biochemistry 15, 5543–5552.PubMedCrossRefGoogle Scholar
- 8.Greene, L. A. (1978) Nerve growth factor prevents the death and stimulates the neuronal differentiation of PC12 pheochromocytoma cells in serum-free medium. J. Cell. Biol. 78, 747–755.PubMedCrossRefGoogle Scholar
- 9.Dichter, M. A., Tischler, A. S., and Greene, L. A. (1977) Nerve growth factorinduced increase in electrical excitability and acetylcholine sensitivity of a rat pheochromocytoma cell line. Nature 268, 501–504.PubMedCrossRefGoogle Scholar
- 10.Su, Y. H., Moxley, M., Kejariwal, R., Mehta, A., Fraser, N. W., and Block, T. M. (2000) The HSV-1 genome in quiescently infected NGF differentiated PC12 cells cannot be stimulated by HSV superinfection. J. Neurovirol. 6, 341–349.PubMedCrossRefGoogle Scholar
Copyright information
© Humana Press Inc. 2005