Abstract
This chapter introduces 13C tracer techniques combined with labeling measurement by mass spectrometry (MS) for the quantification of metabolic pathway fluxes. As an example, the relative contribution of (1) de novo synthesis from glucose and (2) synthesis from building blocks contained in complex substrates to the formation of proteinogenic amino acids in the industrial amino acid producer Corynebacterium glutamicum is investigated.
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Wittmann, C., Heinzle, E. (2005). Metabolic Activity Profiling by 13C Tracer Experiments and Mass Spectrometry in Corynebacterium glutamicum . In: Barredo, JL. (eds) Microbial Processes and Products. Methods in Biotechnology, vol 18. Humana Press. https://doi.org/10.1385/1-59259-847-1:191
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DOI: https://doi.org/10.1385/1-59259-847-1:191
Publisher Name: Humana Press
Print ISBN: 978-1-58829-548-4
Online ISBN: 978-1-59259-847-2
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