Abstract
The field of predictive microbiology is rapidly widening and ranges from bioreactor engineering to the modeling of foodstuff degradation and contamination. In this chapter, we point out how important the choice of experimental conditions is. We also briefly describe some tools (methodology of experimental research) that can be used to design optimal experimental strategies with respect to the study's aim. Emphasis is given on the optimization of microbial metabolite production, with an example showing the screening of factors and another illustrating the use of the response surface methodology.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Addelman, S. (1962) Orthogonal main-effects for asymmetrical factorial experiments. Technometrics, 4, 21–46
Plackett, R. L. and Burman, J. P. (1946) The design of optimum multifactorial experiments. Biometrica 33, 305–332.
Box, G. E. P., Hunter, W. G., and Hunter, J. S. (eds.) (1978) Statistics for Experimenters, Wiley, New York.
Box, G. E. P. and Draper, N. R. (eds.) (1987) Model Building and Response Surfaces, Wiley, New York.
Montgomery, D. C. (1991) Design and Analysis of Experiments, Wiley, New York.
Cochran, W. G. and Cox, G. M. (1950) Experimental Designs, Wiley, New York.
Doehlert, D. H. (1970) Uniform shell designs, Appl. Statist. 19, 231–239.
Cornell, J. A. (1990) Experiments with Mixtures, Wiley, New York.
Scheffe, H. (1958) Experiments with mixtures. J.R. Statist. Soc. B 20, 344–360.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
Vanot, G.E., Sergent, M. (2005). Experimental Design in Microbiology. In: Barredo, JL. (eds) Microbial Processes and Products. Methods in Biotechnology, vol 18. Humana Press. https://doi.org/10.1385/1-59259-847-1:025
Download citation
DOI: https://doi.org/10.1385/1-59259-847-1:025
Publisher Name: Humana Press
Print ISBN: 978-1-58829-548-4
Online ISBN: 978-1-59259-847-2
eBook Packages: Springer Protocols