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Transgenic Plants: Methods and Protocols pp 111–137Cite as

Chloroplast Genetic Engineering to Improve Agronomic Traits

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 286)

Summary

Major crop losses occur annually as a result of biotic and abiotic stresses. The ability to hyperexpress foreign proteins, single-step multigene engineering, lack of positive effect and gene silencing, vector sequences and pleiotropic effects have resulted in several hundred-fold more tolerance to the environmental stresses via chloroplast genetic engineering than nuclear genetic engineering. Maternal inheritance of chloroplast expressed transgenes renders the technology environmentally safe and promotes public acceptance. This review provides protocols for engineering agronomic traits like insect, herbicide and disease resistance; salt and drought tolerance; and phytoremediation via chloroplast genome.

Key Words

  • Disease resistance
  • drought tolerance
  • GM crops
  • herbicide resistance
  • maternal inheritance
  • pest resistance/management
  • phytoremediation
  • plastid transformation
  • transgene containment

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  • DOI: 10.1385/1-59259-827-7:111
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Author information

Authors and Affiliations

  1. Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL

    Henry Daniell, Oscar N. Ruiz & Amit Dhingra

Authors
  1. Henry Daniell
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  2. Oscar N. Ruiz
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  3. Amit Dhingra
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Editor information

Editors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain

    Leandro Peña

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© 2005 Humana Press Inc.

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Daniell, H., Ruiz, O.N., Dhingra, A. (2005). Chloroplast Genetic Engineering to Improve Agronomic Traits. In: Peña, L. (eds) Transgenic Plants: Methods and Protocols. Methods in Molecular Biology™, vol 286. Humana Press. https://doi.org/10.1385/1-59259-827-7:111

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  • DOI: https://doi.org/10.1385/1-59259-827-7:111

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-263-6

  • Online ISBN: 978-1-59259-827-4

  • eBook Packages: Springer Protocols