Abstract
Two-dimensional electrophoresis (2-DE) is a powerful technique to differentially display patterns of protein expression and posttranslational modifications, providing a good strategy to monitor molecular responses induced by the activation or inactivation of specific signaling pathways. In this chapter, optimized protocols for 2-DE using extracts from tissue culture are provided. Protocols for in-gel digestion of gel-resolved proteins, which allow protein identification by mass spectrometry are also discussed.
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© 2004 Humana Press Inc., Totowa, NJ
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Kabuyama, Y., Polvinen, K.K., Resing, K.A., Ahn, N.G. (2004). Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets. In: Dickson, R.C., Mendenhall, M.D. (eds) Signal Transduction Protocols. Methods in Molecular Biology, vol 284. Humana Press. https://doi.org/10.1385/1-59259-816-1:037
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DOI: https://doi.org/10.1385/1-59259-816-1:037
Publisher Name: Humana Press
Print ISBN: 978-1-58829-245-2
Online ISBN: 978-1-59259-816-8
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