Measurement of Apoptotic Cell Clearance In Vitro

  • Andrew Devitt
  • Christopher D. Gregory
Part of the Methods in Molecular Biology book series (MIMB, volume 282)


The phagocytic clearance of apoptotic cells is a highly efficient and non-phlogistic process in vivo. Research in this area has been limited, at least in part, by technical difficulties associated with the techniques used in the detailed study of apoptotic cell clearance mechanisms. This chapter provides details of methods that may be used to study apoptotic cell clearance in vitro. Such methods have been used successfully to identify phagocyte-associated or apoptotic cell-associated molecular players in the recognition process.

Key Words

Macrophage phagocyte apoptotic cell clearance phagocytosis 


  1. 1.
    Devitt, A., Moffatt, O. D., Raykundalia, C., Capra, J. D., Simmons, D. L., and Gregory, C. D. (1998). Human CD14 mediates recognition and phagocytosis of apoptotic cells. Nature 392, 505–509.PubMedCrossRefGoogle Scholar
  2. 2.
    Moffatt, O. D., Devitt, A., Bell, E. D., Simmons, D. L., and Gregory, C. D. (1999). Macrophage recognition of ICAM-3 on apoptotic leukocytes. J. Immunol. 162, 6800–6810.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Andrew Devitt
    • 1
  • Christopher D. Gregory
    • 1
  1. 1.Centre for Inflammation ResearchThe University of Edinburgh Medical SchoolEdinburghUK

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