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Automated NMR Structure Calculation With CYANA

  • Peter Güntert
Part of the Methods in Molecular Biology™ book series (MIMB, volume 278)

Abstract

This chapter gives an introduction to automated nuclear magnetic resonance (NMR) structure calculation with the program CYANA. Given a sufficiently complete list of assigned chemical shifts and one or several lists of cross-peak positions and columes from two-, three-, or four-dimensional nuclear Overhauser effect spectroscopy (NOESY) spectra, the assignment of the NOESY cross-peaks and the three-dimensional structure of the protein in solution can be calculated automatically with CYANA.

Key Words

Protein structure NMR structure determination conformational constraints automated structure determination automated assignment NOESY assignment CYANA program network anchoring constraint combination torsion angle dynamics 

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Peter Güntert
    • 1
  1. 1.RIKEN Genomic Sciences CenterYokohamaJapan

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